Horizontal gene transfer of a genetic island encoding a type III secretion system distributed in Vibrio cholerae |
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| Authors: | Masatomo Morita Shouji Yamamoto Hirotaka Hiyoshi Toshio Kodama Masatoshi Okura Eiji Arakawa Munirul Alam Makoto Ohnishi Hidemasa Izumiya Haruo Watanabe |
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| Affiliation: | 1. Department of Bacteriology I, National Institute of Infectious Diseases, , Tokyo;2. Research Institute for Microbial Diseases, Osaka University, , Osaka;3. National Institute of Animal Health, , Ibaraki, Japan;4. International Center for Diarrhoeal Disease Research, Bangladesh, , Dhaka, Bangladesh |
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| Abstract: | Twelve Vibrio cholerae isolates with genes for a type III secretion system (T3SS) were detected among 110 environmental and 14 clinical isolates. T3SS‐related genes were distributed among the various serogroups and pulsed‐field gel electrophoresis of NotI‐digested genomes showed genetic diversity in these strains. However, the restriction fragment length polymorphism profiles of the T3SS‐related genes had similar patterns. Additionally, naturally competent T3SS‐negative V. cholerae incorporated the ca. 47 kb gene cluster of T3SS, which had been integrated into a site on the chromosome by recombination. Therefore, it is suggested that horizontal gene transfer of T3SS‐related genes occurs among V. cholerae in natural ecosystems. |
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| Keywords: | horizontal gene transfer natural transformation |
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