| Abstract: | Agricultural practices could affect bacterial diversity and community structure by altering soil physical and chemical properties.Straw returning and tillage practices are widely used in agriculture,however,the effects of these agricultural practices on microbiomes are still unclear.In the present study,we compared the 18 bacterial communities of soil with different straw retu rning and tillage treatment combinations.The V3-V4 regions of the 16 S ribosomal RNA were amplified and analyzed by high-throughput sequencing technology.The results showed that the bacterial communities were consistently dominated by Acidobacteria,Proteobacteria,Actinobacteria,and Chloroflexi.Short-term straw returning and tillage practices significantly altered the diversity,relative abundance and functions of the soil microbiome.Soil subjected to rotary tillage and straw re turning(RTS)combination possessed the highest bacterial diversity and lowest ratio of G+/G-bacteria,indicating that RTS could be an efficient integrated manageme nt system to improve microbiome in the short term.Double verifications based on relative abundance and network analysis,revealed close relationships of Mycobacterium and Methylibium with RTS,indicating they could serve as biomarkers for RTS.Investigating microbial changes under different agricultural practices will provide valuable foundations for land sustainable utilization and increase crop yields. |
