Construction, Characterization, and Chromosomal Mapping of a Fosmid Library of the White-Cheeked Gibbon (Nomascus leucogenys) |
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作者单位: | Liping Chen(Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China;Graduate School of Chinese Academy of Sciences, Beijing 100039, China) ;
Jianping Ye(Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China;Graduate School of Chinese Academy of Sciences, Beijing 100039, China) ;
Yan Liu(Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China;Graduate School of Chinese Academy of Sciences, Beijing 100039, China) ;
Jinghuan Wang(Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China) ;
Weiting Su(Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China) ;
Fengtang Yang(Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK) ;
Wenhui Nie(Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China) ; |
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摘 要: |
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关 键 词: | 染色体 荧光性 杂交 基因 |
Construction, Characterization, and Chromosomal Mapping of a Fosmid Library of the White-Cheeked Gibbon (Nomascus leucogenys) |
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Authors: | Liping Chen Jianping Ye Yan Liu Jinghuan Wang Weiting Su Fengtang Yang Wenhui Nie |
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Institution: | [1]Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China [2]Graduate School of Chinese Academy of Sciences, Beijing 100039, China |
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Abstract: | Gibbons have experienced extensive karyotype rearrangements during evolution and represent an ideal model for studying the underlying molecular mechanism of evolutionary chromosomal rearrangements. It is anticipated that the cloning and sequence characterization of evolutionary chromosomal breakpoints will provide vital insights into the molecular force that has driven such a radical karyotype reshuffle in gibbons. We constructed and characterized a high-quality fosmid li- brary of the white-cheeked gibbon (Nomascus leucogenys) containing 192,000 non- redundant clones with an average insert size of 38 kb and 2.5-fold genome coverage. By end sequencing of 100 randomly selected fosmid clones, we generated 196 se- quence tags for the library. These end-sequenced fosmid clones were then mapped onto the chromosomes of the white-cheeked gibbon by fluorescence in situ hy- bridization, and no spurious chimeric clone was detected. BLAST search against the human genome showed a good correlation between the number of hit clones and the number of chromosomes, an indication of unbiased chromosomal distribu- tion of the fosmid library. The chromosomal distribution of the mapped clones is also consistent with the BLAST search result against human and white-cheeked gibbon genomes. The fosmid library and the mapped clones will serve as a valu- able resource for further studying gibbons' chromosomal rearrangements and the underlying molecular mechanism as well as for comparative genomic study in the lesser apes. |
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Keywords: | fosmid library white-cheeked gibbon chromosomal mapping fluorescence in situ hybridization whnie@mail kiz ac cn Construction Characterization Library study apes serve able resource distribution consistent result human genome genomes indication BLAST search good correlation number spurious |
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