Cloning,molecular characterization,and expression analysis of Copine 8 |
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Authors: | Maitra Rangan Grigoryev Dmitry N Bera Tapan Kumar Pastan Ira H Lee Byungkook |
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Institution: | Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 37 Convent Drive Room 5120 MSC 4262, Bethesda, MD 20892-4264, USA. |
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Abstract: | Copines are ubiquitously expressed, phospholipid-binding proteins that have been conserved through evolution. In this paper, we report the cloning and molecular characterization of a new member of the Copine family, Copine 8. This gene has been isolated and characterized using a combination of bioinformatic and experimental approaches. Using an algorithm to cluster ESTs (expressed sequence tags) that are available through the public "GoldenPath" database, Copine 8 was initially identified as a gene predominantly expressed in prostate and testis. Cloning and molecular analysis revealed that this gene is expressed in low-levels in most tissues examined. Two different isoforms of this gene have been isolated. Strongest expression of Copine 8 mRNA is seen in the prostate, heart, and brain. Taken together, this data suggest that Copine 8 may have an important role to play in prostate regulation and development. |
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Keywords: | Copine Cloning Functional genomics Expression analysis Isoforms Prostate |
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