Sensitivity improvement in 2D and 3D HCCH spectroscopy using heteronuclear cross-polarization |
| |
Authors: | Ananya Majumdar Hong Wang Robert C. Morshauser Erik R. P. Zuiderweg |
| |
Affiliation: | (1) Biophysics Research Division, The University of Michigan, 930 North University, 48109-1055 Ann Arbor, MI, U.S.A. |
| |
Abstract: | Summary A new method, which employs a sequence of heteronuclear-homonuclear-heteronuclear Hartmann-Hahn (HEHOHEHAHA) cross-polarization steps for obtaining through-bond H-C-C-H correlations in larger proteins (Mr > 15 kDa), is presented. The method has significantly higher sensitivity compared to INEPTHOHAHA-INEPT-based techniques. An additional feature of this experiment is that well-phaseable spectra may be obtained with a minimal (4-step) phase cycle and, consequently, experimental time can be utilized towards obtaining high resolution in indirect dimensions. Results from 2D and 3D HEHOHEHAHA experiments on T4-lysozyme are presented. |
| |
Keywords: | Heteronuclear cross polarization HCCH HOHAHA TOCSY T4-lysozyme Labeled proteins Composite pulse sequences |
本文献已被 SpringerLink 等数据库收录! |