The boundaries and copy numbers of Ti plasmid T-DNA vary in crown gall tumors |
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| Authors: | D J Merlo R C Nutter A L Montoya D J Garfinkel M H Drummond M D Chilton M P Gordon E W Nester |
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| Affiliation: | (1) Department of Microbiology and Immunology, University of Washington, 98195 Seattle, Washington, USA;(2) Department of Biochemistry, University of Washington, 98195 Seattle, Washington, USA;(3) Present address: Division of Biological Sciences, University of Missouri, 65211 Columbia, Missouri, USA |
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| Abstract: | Summary The Ti plasmid DNA maintained in octopine-type crown gall tumor lines is variable, but always includes at least part of the Ti plasmid that maps over the region of Hind III fragment 1 of pTi-B6-806. The right-hand boundary of transferred DNA (T-DNA) varies considerably among the three independent tumor lines examined; the left boundary was not located definitively. The T-DNA of two sibling clones of the same tumor line, E1 and E9, appears identical. The copy number of T-DNA in E9 tumor DNA appears higher for the right end (about 30 copies) than for the left end (approximately 1 copy). |
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