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Altered restriction of nuclear RNA during incubation in vitro
Authors:G A Clawson  E A Smuckler
Institution:Department of Pathology, University of California School of Medicine, San Francisco, California 94143 USA
Abstract:Nuclei were isolated from rat liver and incubated invitro in two commonly employed RNA transport assays. Released 14C] RNA was isolated and hybridized with filter-bound DNA in the presence of competing cytoplasmic RNA. A significant portion of RNA which was transported to either medium was not represented in cytoplasmic RNA. These results indicate that the restriction of some sequences to the nucleus invivo is not maintained invitro.
Keywords:To whom all correspondence should be addressed  
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