Polyamine induced changes in the ADP-ribosylation of nuclear proteins from rat liver |
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Authors: | F W Perrella M A Lea |
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Institution: | Department of Biochemistry New Jersey Medical School Newark, N. J. 07103 USA |
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Abstract: | Purified rat liver nuclei were incubated with 3H]NAD. Altered patterns of ADP-ribosylation of nuclear proteins occurred with 1 mM spermidine or spermine with the latter polyamine causing the greater change. Spermine treated nuclei showed a two-fold increase in ADP-ribose incorporation into H1 histones and a decrease in the other histones. Likewise, the incorporation into the more acidic non-histone nuclear proteins was greater with spermine than spermidine. These results suggest that polyamines may exert a regulatory function by altering the pattern of ADP-ribosylation of both histone and non-histone nuclear proteins. |
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