DNA extraction from a previously recalcitrant plant genus |
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| Authors: | David M. Webb Steven J. Knapp |
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| Affiliation: | (1) Department of Crop Science, Oregon State University, 97331 Corvallis, Oregon, USA |
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| Abstract: | Numerous DNA extraction methods failed to remove contaminants that interfere with restriction digests ofCuphea DNA. The method described here removes those contaminants and maintains relatively high DNA yields. The primary purification process consists of washing the DNA with phenol while it is complexed with CTAB and dissolved in 1 M NaCl. |
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| Keywords: | DNA extraction Cuphea CTAB precipitation phenol |
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