Alternative, cost-effective fungus-staining method for control slides in cytology and histopathology |
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Authors: | da Silva Vinicius Duval |
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Institution: | Department of Pathology and Radiation, Faculty of Medicine, Pontifícia Universidade Catolica do Rio Grande do Sul, Hospital Sao Lucas, Porto Alegre, RS, Brazil. vinids@pucrs.br |
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Abstract: | OBJECTIVE: To develop a cost-effective, reliable and safe method of providing fungal control slides for routine use in pathology laboratories. STUDY DESIGN: A set of easily available, low-cost material was tested to obtain fungal colonies on substrate adequate for paraffin-embedded sections or smears. RESULTS: Such material as cheese is a simple, inexpensive and practical culture medium for silver-positive fungi. A batch of paraffin blocks can be prepared to maintain a stock of control material in the laboratory. CONCLUSION: It is useful to maintain fungal colonies to produce staining control specimens using small pieces of refrigerated cheese to easily produce silver-staining control specimens or smears embedded in paraffin, reducing the risk of accidental exposure to potentially infective pathogens in the laboratory. This method might also be a good alternative for conserving routine surgical specimens, considering the currently decreasing numbers of necropsy and large specimens, particularly from immunosuppressed and infected patients. |
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