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A simple method for preparation of D-rhamnose |
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| Authors: | Ramm Michael Lobe Martina Hamburger Matthias |
| Affiliation: | Institute of Pharmacy, Friedrich-Schiller-University Jena, Semmelweisstrasse 10, D-07743 Jena, Germany. mramm@pmail.hki-jena.de |
| Abstract: | A rapid procedure for the preparation of D-rhamnose from bacterial lipopolysaccharide (LPS) has been developed. It involves purification of LPS from Pseudomonas syringae pv. phaseolicola by phenol extraction and hydrophobic interaction chromatography (HIC), followed by mild hydrolysis and cleavage of the O-antigen into D-fucose and D-rhamnose. The monosaccharides were separated by column chromatography, and D-rhamnose recovered after filtration over Sephadex-LH 20. |
| Keywords: | smallcaps" >d-Rhamnose 6-Deoxyhexose Pseudomonas syringae pv. phaseolicola Lipopolysaccharide Endotoxin Hydrophobic interaction chromatography Column chromatography |
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