| RNase P核酶对人巨细胞病毒UL54基因mRNA体外切割作用 |
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| 引用本文： | 周乐平,李弘剑,陈浩军,李月琴,何华坤,王波,周天鸿. RNase P核酶对人巨细胞病毒UL54基因mRNA体外切割作用[J]. Virologica Sinica, 2004, 19(2): 110-113 |
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| 作者姓名： | 周乐平 李弘剑 陈浩军 李月琴 何华坤 王波 周天鸿 |
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| 作者单位： | 暨南大学生命科学技术学院,广东广州510632 |
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| 基金项目： | 国家自然科学基金(30370776)，广东省自然科学基金重大项目(36703)，广东省自然科学基金(021162,000718) |
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| 摘 要： | 外部引导序列(EGSs)是mRNA靶序列互补并引导RNaseP切割的小RNA片段。我们设计与人巨细胞病毒HCMV(Human Cytomegalovirus)UL54基因mRNA序列互补的EGSs，将其与大肠杆菌来源RNaseP催化核心M1RNA构建成M1GS核酶。通过对UL54基因亚克降片转录产物体外切割研究，证实该核酶具备对UL54 mRNA片段的特异切割能力，可以发展成为一种抗病毒试剂。
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| 关 键 词： | 人巨细胞病毒HCMV UL54基因 mRNA RNaseP 外部引导序列 |
Targeted in vitro Cleavage of Human Cytomegalovirus UL54 mRNA Segments by RNase P |
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| ZHOU Le-ping,LI Hong-jian,CHEN Hao-jun,LI Yue-qin,HE Hua-kun,WANG Bo,ZHOU Tian-hong. Targeted in vitro Cleavage of Human Cytomegalovirus UL54 mRNA Segments by RNase P[J]. 中国病毒学(英文版), 2004, 19(2): 110-113 |
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| Authors: | ZHOU Le-ping LI Hong-jian CHEN Hao-jun LI Yue-qin HE Hua-kun WANG Bo ZHOU Tian-hong |
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| Affiliation: | ZHOU Le-ping,LI Hong-jian,CHEN Hao-jun,LI Yue-qin,HE Hua-kun,WANG Bo,ZHOU Tian-hong** |
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| Abstract: | External guide sequences (EGSs) are oligonucleotides that consist of a sequence com- plementary to a target RNA and specific cleavage occurs at such locations acted by M1 RNA, the catalytic RNA subunit of RNase P from Escherichia coli. A sequence-specific ribozymes (M1GS-C6 RNA) derived from EGSs and M1 RNA were used to target a region of UL54 mRNA encoding major DNA polymerase of Human Cytomegalovirus in vitro. The results showed that M1GS ribozyme can function as an antiviral agent and effectively cleave UL54 mRNA. |
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| Keywords: | Human Cytomegalovirus(HCMV) UL54 RNase P External Guide Sequences (EGSs) |
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