The in vitro effect of methylprednisolone on the processes of activation of CD4+CD45RO+ T-cells from healthy subjects and rheumatoid-arthritis patients |
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Authors: | N. M. Todosenko O. G. Khaziakhmatova K. A. Yurova I. P. Malinina L. S. Litvinova |
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Affiliation: | 1.Laboratory of Immunology and Cell Biotechnology,Immanuel Kant Baltic Federal University,Kaliningrad,Russia;2.Rheumatology Department,Regional Clinical Hospital,Kaliningrad,Russia |
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Abstract: | Flow cytometry has been used to analyze the changes in the number of CD4+ cells that expressed surface markers of activation (CD25, CD71, HLA-DR, and CD95) in cultures of TCR-stimulated CD3+CD45RO+ Т-lymphocytes after in vivo exposure to different concentrations of methylprednisolone (MP). T-cells were obtained from healthy donors and rheumatoid arthritis (RA) patients. Suppressive action of МР on the expression of activation and proliferation markers (CD25 and CD71, respectively) by CD4+ T-cells was observed in all study subjects. МР increased the number of CD4+ HLA-DR+/CD95+ cells among the СD3+CD45RO+ cells obtained from RA patients and subjected to TCR activation, whereas the number of such cells in the control group decreased after MP treatment. The MP-induced changes in the cells subjected to TCR activation can be indicative of relative resistance of the CD4+CD45RO+HLA-DR+/CD95+ cell population in RA patients to the action of glucocorticoids and the possible role of this subpopulation in RA pathogenesis. |
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