Correlation of O2 uptake rate and mitochondrial activity in carrot cell cultures exposed to laminar fluid shear |
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| Authors: | Victor Wong David Williams Christopher Colby David A. Saint |
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| Affiliation: | (1) Department of Chemical Engineering, Adelaide University, Adelaide, SA, 5005, Australia;(2) Department of Physiology, Adelaide University, Adelaide, SA, 5005, Australia |
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| Abstract: | Measuring uncoupled oxygen uptake rate (OUR) could provide a convenient method for quantifying changes in the metabolic activity of plant cultures caused by hydrodynamic shear. Experiments on Daucus carota (carrot) cells were performed in a novel O2-permeable Couette viscometer at varying levels of laminar shear (6 to 100 N m–2). When the uncoupled OUR of the cells was compared with mitochondrial activity (determined by 2,3,5-triphenyl tetrazolium chloride assay), a significant correlation was observed (R=0.91 by linear regression). |
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| Keywords: | laminar fluid shear oxygen uptake rate plant culture sub-lytic cellular response |
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