Measurement of zinc, copper, manganese, and iron concentrations in hair of pituitary dwarfism patients using flameless atomic absorption spectrophotometry |
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Authors: | Fumie Miki Takashi Sakai Masanobu Wariishi Masayuki Kaji |
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Institution: | (1) Faculty of Human Life Science, Shikoku University, Furukawa, Ojin-cho, Tokushima, Japan;(2) Department of Science for Living, Shikoku University Junior College, Furukawa, Ojin-cho, Tokushima, Japan;(3) Division of Endocrinology and Metabolism, Shizuoka Children’s Hospital, Urushiyama, Shizuoka, Japan |
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Abstract: | Pituitary dwarfism (hGHD) is known to be associated with trace element deficiency, which causes improper functioning of the
involved endocrine system. Previously, we reported on the head hair concentrations of zinc, copper, manganese, and iron from
a total of 418 normal subjects (154 male and 264 female). In this report, we analyzed the head hair concentrations of the
same four trace metals of 103 hGHD children (60 male and 43 female) under treatment with human growth hormone (hGH). These
subjects ranged in age from 5 to 18 yr. The results were compared with 338 agematched normal subjects (120 male and 218 female).
Both male and female hGHD showed approx 1.7 times higher zinc concentrations than normal subjects. Cheruvanky et al. reported
a similar trend but with a slightly lower difference between hGHD and normal subjects.
The average copper content in the hair of both male and female subjects also showed higher values for the hGHD than for the
normal subjects, a trend similar to the values reported by Teraoka et al. In the case of manganese, the concentrations in
hair of the hGHD were approx 50% of the values in the normal subjects. Head hair concentrations of iron in the hGHD were commensurate
with the normal subjects.
Because the content of trace elements in hair varies with the age of subjects, as a control, we investigated the head hair
concentration of zinc from 20 healthy girls ranging in age from 10 to 18 yr. The average zinc concentration decreased from
10 to 12 yr, but no clear relation to age was observed from 13 yr and older. These trends were similar to our previous report.
The zinc concentration in hair and body weight gain over a year was negatively correlated.
The age variation in the content of zinc, copper, manganese, and iron in hair was measured comparing hGHD with the normal
subjects in various ages. Concerning the zinc-level variation of hGHD and normal subjects, there were conspicuous differences
between hGHD and normal subjects. For copper, the variations in concentration with age were similar to zinc. Regarding the
age variations for manganese, hGHD had lower concentrations in hair compared to the normal subjects throughout adolescence
(11–18 yr).
We have studied the effects between the hair and these trace element concentrations in hGHD before and after hGH administration.
These results suggest that hGH affects the metabolism of these trace elements. |
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Keywords: | Pituitary dwarfism (hGHD) zinc copper manganese iron flameless atomic absorption spectrophotometry |
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