Hydrolysis of Heparin by the Immobilized Enzymatic Complex from Streptomyces kurssanovii |
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| Authors: | Bannikova G. E. Stolbushkina P. P. Drozd N. N. Vikhoreva G. A. Varlamov V. P. Makarov V. A. Panov A. V. |
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| Affiliation: | (1) Kosygin Moscow State Textile University, Malaya Kaluzhskaya ul. 1, Moscow, 119991, Russia;(2) Hematology Research Center, Russian Academy of Medical Sciences, Moscow, 125167, Russia;(3) Bioengineering Center, Russian Academy of Sciences, Moscow, 117312, Russia;(4) Mendeleev Russian University of Chemistry and Technology, Moscow, 125820, Russia |
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| Abstract: | The possibility of obtaining low-molecular-weight heparins using a chitinolytic enzymatic complex immobilized on Silochrom has been demonstrated. The optimal conditions of this process (sodium acetate buffer, pH 7.0–7.5; temperature, 40–45°C; and duration of hydrolysis, 3 h) were determined. Depending on the ratio between heparin and the immobilized enzymatic complex, samples with molecular weights varying from 1.7 to 4.7 kDa, were obtained. These complexes inhibited factor Xa 2.0–3.7 times more effectively than the original heparin. |
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