中国汉族人群DNA酶I基因多态性与不稳定性心绞痛的关系

目的：探讨脱氧核糖核酸酶（IDNA酶I）基因多态性与汉族人群不稳定性心绞痛（unstable angina pectoris,UAP）易感性的关系。方法：以196例UAP患者为病例组,排除冠心病的297例体检者为对照组,应用PCR及PCR-限制性片段长度多态（PCR-RFLP）分析DNA酶I基因8外显子单核苷酸多态位点A2317G及4内含子56bp可变串联重复序列（HumDN1）多态性;协方差分析A2317G、HumDN1各基因型与UAP患者血脂的关系,将年龄、性别、高血压、糖尿病及吸烟作为协变量;x2检验分析UAP患者冠脉血管病变支数与DNA酶I基因型的关系。结果：UAP组与对照组A2317G、HumDN1各基因型及等位基因分布无明显统计学差异（P〉0.05）,两组DNA酶I单体型分布亦无差异。UAP患者DNA酶I各基因型血脂水平、冠脉血管病变支数的差异无明显统计学意义,所有P值均〉0.05。结论：DNA酶I基因多态性与中国汉族人群不稳定心绞痛及其血脂水平无明显相关性。

Association of Deoxyribonuclease I Genetic Polymorphisms with Unstable Angina Pectoris in Han Chinese

Objective：To investigate the relationship between deoxyribonuclease I gene polymorphisms and unstable angina pectoris（UAP） in Han Chinese population.Methods：A2317G and HumDN1 polymorphisms in 196 UAP patients and 297 unrelated controls were detected by PCR and PCR-restriction fragment length polymorphism.Analysis of covariance was used to analyze the relationship between genotypes and serum lipids,with age,sex,hypertension,diabetes and smoking as covariates.The relationship between UAP genotypes and coronary lesion vessels was tested by x2 analysis.Results：Genotype distributions and allele frequencies of A2317G and HumDN1 did not differ between UAP patients and control group（all P0.05）.None of estimated haplotypes significantly increased or decreased the risk of UAP.In analysis of covariance,plasma lipids were not associated with deoxyribonuclease I genotypes in UAP patients（all P0.05）.Different UAP genotypes shared the same coronary lesion vessels.Conclusions：Deoxyribonuclease I gene polymorphisms are not associated with susceptibility to UAP and plasma lipids levels in Han Chinese population.