A study of acid phosphatase in mouse kidney using naphthol AS/BI phosphate as a substrate |
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| Authors: | Viviane Maggi Wai Lam Chng |
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| Institution: | (1) Tissue and Organ Culture Unit, Imperial Cancer Research Fund, W.C.2 London |
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| Abstract: | Summary A kinetic study of mouse kidney acid phosphatase has been performed using an application of the histochemical method ofBurstone (1958a, b). The suitability of the use of naphthol AS/BI phosphate as a substrate for biochemical assays of acid phosphatase has been ascertained. However, the rate of inhibition of the enzyme by sodium molybdate and sodium fluoride suggests that naphthol AS/BI phosphate may represent a substrate for an acid phosphatase different from -glycerophosphatase. |
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