Antigenic Pattern of Human Brain Glycoproteins as Described by Monoclonal Antibodies |
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Authors: | Erik Berglund Per Almqvist Hans Lindroos Sven Carlsson Torgny Stigbrand |
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Institution: | Department of Physiological Chemistry, University of Umeå, Umeå, Sweden |
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Abstract: | A battery of monoclonal antibodies was raised against a preparation of lentil lectin-binding membrane glycoproteins from human brain. Out of 26 established hybridomas, nine produced antibodies against the human Thy-1 antigen. For the remaining 17 lines, reactivity with at least six other antigens could be identified after immunoprecipitation and immunoblotting. Several of the antigens were di- or trimeric, mainly in the molecular weight range of 60-120 kDa. Two of the antibodies were reactive with high-molecular-weight aggregates and four targets for the antibody reactivity were not identifiable by immunoprecipitation of iodinated antigens. Three of the identified antigens were shown by quantitative enzyme-linked immunosorbent assay tests on various human tissues to be specifically expressed in the brain. |
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Keywords: | Human brain Glycoproteins Thy-1 Monoclonal antibodies) |
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