The distribution and colocalization of neuropeptides and 5-hydroxytryptamine in pelvic nerves supplying the posterior large intestine of the toad,Bufo marinus |
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Authors: | Philip J Davies Peregrine B Osborne Graeme Campbell |
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Institution: | (1) Department of Zoology, University of Melbourne, 3052 Parkville, Victoria, Australia;(2) Present address: Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, 97201 Portland, OR, USA;(3) Present address: Department of Physiology, Monash University, 3168 Clayton, Victoria, Australia |
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Abstract: | The distribution and colocalization of neuropeptides and 5-hydroxytryptamine in the posterior portion of the large intestine of the toad was studied using single- and dual-label immunohistochemistry. Neurons containing colocalized galanin/somatostatin or vasoactive intestinal peptide alone were observed along intramural pelvic nerves. Some of the galanin/somatostatin neurons also contained 5-hydroxytryptamine. Synaptic boutons containing colocalized calcitonin gene-related peptide/vasoactive intestinal peptide were associated with the galanin/somatostatin neurons. The muscle of the large intestine was also innervated by axons containing galamin/somatostatin, vasoactive intestinal peptide/calcitonin gene-related peptide or vasoactive intestinal peptide alone. Nerve fibres containing calcitonin gene-related peptide/substance P, probably representing primary afferent nerves, were also associated with muscle bundles. Submucosal blood vessels carried dense plexuses of fibres containing vasoactive intestinal peptide alone or and calcitonin gene-related peptide/substance P. Adrenergic perivascular nerves also contained galanin and neuropeptide Y. |
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Keywords: | Immunohistochemistry Pelvic nerves Neuropeptides Large intestine Bufo marinus (Anura) |
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