| Abstract: | A chloral hydrate-containing tube-gel electrophoresis system previously described by Ballou, Sundharadas, and Bach (1974) Science 185, 531] permitted charge and size separation of membrane proteins, but was difficult to use and inapplicable to slab gels. A greatly simplified photopolymerized chloral hydrate-polyacrylamide gel electrophoresis system is presented here for use with tube or slab containers. An aluminum lactate buffer system provides good solubilization and resolution. Its advantages over the previously described system are simplicity and multiple sample capacity. |
