Localization of protein phosphokinase activities in the nucleolus distinct from extra-nucleolar regions in rat ventral prostate nuclei |
| |
Authors: | M. J. Wilson K. Ahmed |
| |
Affiliation: | Department of Cell Biology, Roche Institute of Molecular Biology, Nutley, N.J. 07110, USA |
| |
Abstract: | Rat ventral prostate nucleoli contain protein phosphokinase(s) which have distinctly different characteristics from protein phosphokinase(s) localized in the extra-nucleolar regions of the nucleus. The differences pertain to pH vs activity profiles and activation by divalent cations, utilizing dephospho-phosvitin as substrate. Lysine-rich and arginine-rich F3 histones are also phosphorylated by nucleolar protein phosphokinase(s). Phosphorylation of histones by either nucleolar or extra-nucleolar fractions was not stimulated by cAMP, whereas that of phosvitin was slightly inhibited. |
| |
Keywords: | Reprint requests to: Michael J. Wilson Toxicology Research Laboratory Veterans Administration Hospital 54th Street and 48th Avenue South Minneapolis MN 55417 USA. |
本文献已被 ScienceDirect 等数据库收录! |