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Identification and characterization of polymorphic microsatellite loci in the blue shark Prionace glauca, and cross-amplification in other shark species
Authors:Mendonça F F  Ussami L H F  Hashimoto D T  Pereira L H G  Porto-Foresti F  Oliveira C  Gadig O B F  Foresti F
Institution:1. Laboratório de Biologia e Genética de Peixes, Departamento de Morfologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista, CEP 18618‐000, S?o Paulo, Brazil;2. Laboratório de Genética de Peixes, Departamento de Biologia, Faculdade de Ciências, Universidade Estadual Paulista, CEP 17033‐360, S?o Paulo, Brazil;3. Laboratório de Pesquisa em Elasmobranquios, Campus Experimental do Litoral Paulista, Universidade Estadual Paulista, CEP 11330‐900, S?o Paulo, Brazil
Abstract:Two to 14 alleles were found to be segregating per locus (mean 5·2), with observed and expected heterozygosities ranging from 0·08 to 0·78 and 0·08 to 0·94, respectively. Cross-amplification of six of these microsatellite loci indicated that they are also polymorphic in three species of Carcharhiniformes and two species of Lamniformes. The newly developed primers reported here constitute a useful tool for genetic population analyses on Prionace glauca and, potentially, other related species.
Keywords:conservation  elasmobranch  genetic variation  marine ecology  polymorphism  population structure
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