Hemoglobin attenuates the effects of inspired oxygen on plasma isofurans in humans during upper-limb surgery |
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Authors: | Tomas B. CorcoranAnne E. Barden Emilie Mas Sina GrapeViktoria Koren Michael PhillipsL. Jackson Roberts II Trevor A. Mori |
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Affiliation: | a Department of Anaesthesia & Pain Medicine, Royal Perth Hospital, Perth, WA 6847, Australiab School of Medicine and Pharmacology, University of Western Australia, Perth, WA 6847, Australiac The Cardiovascular Research Centre, Perth, WA, Australiad Western Australian Institute for Medical Research, University of Western Australia, Perth, WA 6847, Australiae Department of Pharmacology, Vanderbilt University, Nashville, TN 37240, USA |
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Abstract: | Reperfusion injury is characterized by significant oxidative stress. F2-isoprostanes (F2-IsoP's) and isofurans (IsoF's), the latter preferentially produced during increased oxygen tension, are recognized markers of in vivo oxidative stress. We aimed to determine whether increasing oxygen tension during reperfusion modified levels of plasma total IsoF's and F2-IsoP's. Forty-five patients undergoing upper-limb surgery were randomized to receive inspired oxygen concentrations of 30, 50, or 80% during the last 15 min of surgery. Venous blood samples were taken before the change in inspired oxygen, after 10 min (before reperfusion), and after 15 min (5 min after reperfusion). IsoF's and F2-IsoP's were measured by gas chromatography-mass spectrometry. Venous oxygen tension and hemoglobin concentrations were also measured. Plasma IsoF and F2-IsoP levels in the 50 and 80% O2 groups were not significantly different from those of the 30% O2 group. In secondary analyses, using data combining all groups, levels of IsoF's, but not F2-IsoP's, associated with higher venous oxygen tension (P = 0.038). Hemoglobin negatively modified the influence of oxygen tension on levels of IsoF's (P = 0.014). This study has shown, for the first time, that plasma IsoF levels associate with higher oxygen tension in a human model of reperfusion, and this effect is significantly attenuated by hemoglobin. |
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Keywords: | AA, arachidonic acid F2-IsoP, F2-isoprostane GCMS, gas chromatography-mass spectrometry HPLC, high-performance liquid chromatography IsoF, isofuran PvO2, oxygen tension/venous oxygen partial pressure |
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