Visualization of a rapid,red/far-red light-dependent reaction by centrifuge microscopy |
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Authors: | S Takagi E Kamitsubo R Nagai |
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Institution: | (1) Department of Biology, College of General Education, Osaka University, Machikaneyama 1-1, Toyonaka, 560 Osaka, Japan;(2) Biological Laboratory, Hitotsubashi University, Kunitachi, Tokyo |
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Abstract: | Summary Using a centrifuge microscope with stroboscopic illumination, we examined the effects of light irradiation on the passive movement of chloroplasts in dark-adapted mesophyll cells ofVallisneria gigantea. While irradiation with red light accelerates the passive gliding of chloroplasts produced by centrifugal force, irradiation with far-red light negates this effect. Irradiation with blue light does not accelerate the passive gliding, while red light is completely effective even in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea, an inhibitor of photosynthesis. An apparently active movement of chloroplasts can be induced by irradiation with red or blue light only in the presence of the far-red light-absorbing form of phytochrome. The significance of the reaction in the light with respect to the regulation of cytoplasmic streaming is discussed.Abbreviations APW
artificial pond water
- CMS
centrifuge microscope of the stroboscopic type
- DCMU
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- Pfr
phytochrome, far-red light-absorbing form
- Pr
phytochrome, red light-absorbing form |
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Keywords: | Centrifuge microscopy Cytoplasmic streaming Far-red light Mesophyll cells Red light Vallisneria |
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