| Abstract: | The mechanics by which normal human erythrocytes join on a plastic cover slip into two cell doublets and larger aggregates of rouleaux were studied microscopically. Polyvinylpyrrolidone (PVP-360) or dextran (DX-70 or DX-110) were used as the rouleau agents. The minimum concentration of the rouleau-inducing agents required to form doublets was 1 g/L for PVP-360 and 5 g/L for the DXs. Three modes of interaction were observed in Ringer's solution with PVP or DX, cresting and flipping (involving no intercellular sliding) and a sliding mode of doublet formation (involving less gravitational work and less cellular deformation). The sliding mechanism occurred in suspensions with the lower concentrations of the rouleau agent but was also observed when geometric constraints prevented the nonsliding interaction of larger groups of cells in the higher concentrations of the rouleau agent. The technique provides a sensitive index for studying the combined effect of cellular flexibility and intercellular adhesion. Significant changes were observed for reduced membrane surface charge or reduced ionic calcium. |
