Seasonal carbon allocation to arbuscular mycorrhizal fungi assessed by microscopic examination, stable isotope probing and fatty acid analysis |
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Authors: | Ylva Lekberg Søren Rosendahl Anders Michelsen Pål Axel Olsson |
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Institution: | 1. Department of Biology, University of Copenhagen, ?ster Farimagsgade 2D, DK 1353, Copenhagen K, Denmark 2. MPG Ranch and Department of Ecosystem and Conservation Sciences, University of Montana, 1001 S. Higgins Ave, Suite A3, Missoula, MT, 59802, USA 3. Biodiversity, Department of Biology, Lund University, Ecology Building, SE-223 62, Lund, Sweden
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Abstract: | Background and Aim Climate change models are limited by lack of baseline data, in particular carbon (C) allocation to – and dynamics within – soil microbial communities. We quantified seasonal C-assimilation and allocation by plants, and assessed how well this corresponds with intraradical arbuscular mycorrhizal fungal (AMF) storage and structural lipids (16:1ω5 NLFA and PLFA, respectively), as well as microscopic assessments of AMF root colonization. Methods Coastal Hypochoeris radicata plants were labeled with 13CO2 in February, July and October, and 13C-allocation to fine roots and NLFA 16:1ω5, as well as overall lipid contents and AM colonization were quantified. Results C-allocation to fine roots and AMF storage lipids differed seasonally and mirrored plant C-assimilation, whereas AMF structural lipids and AM colonization showed no seasonal variation, and root colonization exceeded 80 % throughout the year. Molecular analyzes of the large subunit rDNA gene indicated no seasonal AMF community shifts. Conclusions Plants allocated C to AMF even at temperatures close to freezing, and fungal structures persisted in roots during times of low C-allocation. The lack of seasonal differences in PLFA and AM colonization indicates that NLFA analyses should be used to estimate fungal C-status. The implication of our findings for AM function is discussed. |
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