Transgenic American elm shows reduced Dutch elm disease symptoms and normal mycorrhizal colonization |
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Authors: | Andrew E Newhouse Franziska Schrodt Haiying Liang Charles A Maynard William A Powell |
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Institution: | (1) Faculty of Environmental and Forest Biology, SUNY College of Environmental Science and Forestry, Syracuse, NY, USA;(2) Department of Genetics and Biochemistry, Clemson University, Clemson, SC, USA;(3) Faculty of Forest and Natural Resources Management, SUNY College of Environmental Science and Forestry, Syracuse, NY, USA |
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Abstract: | The American elm (Ulmus americana L.) was once one of the most common urban trees in eastern North America until Dutch-elm disease (DED), caused by the fungus
Ophiostoma novo-ulmi, eliminated most of the mature trees. To enhance DED resistance, Agrobacterium was used to transform American elm with a transgene encoding the synthetic antimicrobial peptide ESF39A, driven by a vascular
promoter from American chestnut. Four unique, single-copy transgenic lines were produced and regenerated into whole plants.
These lines showed less wilting and significantly less sapwood staining than non-transformed controls after O. novo-ulmi inoculation. Preliminary observations indicated that mycorrhizal colonization was not significantly different between transgenic
and wild-type trees. Although the trees tested were too young to ensure stable resistance was achieved, these results indicate
that transgenes encoding antimicrobial peptides reduce DED symptoms and therefore hold promise for enhancing pathogen resistance
in American elm. |
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Keywords: | Ulmus americana Ophiostoma novo-ulmi Dutch elm disease resistance Transgenic Mycorrhizae |
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