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Diversity of Saccharomyces strains in wine fermentations: analysis for two consecutive years
Authors:Sabate  Cano  Querol  & Guillamón
Institution:Unidad de Microbiologia, Facultad de Medicina, Universidad Rovira i Virgili, Reus, Tarragona,;Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (C.S.I.C.), Paterna, Valencia,;Departamento de Bioquímica y Biotecnología, Facultad de Enología, Universidad Rovira i Virgili, Tarragona, Spain
Abstract:An ecological study of Saccharomyces cerevisiae strains in spontaneous alcoholic fermentation has been conducted in the same winery for two consecutive years (1994 and 1995). Yeast cells were identified and characterized using mitochondrial DNA restriction analysis. Although a great diversity of wild strains was observed, a sequential substitution of S. cerevisiae strains during the different phases of fermentation was detected. Furthermore, the most frequent strains were encountered in both years, and the dynamic populations were not influenced by climatic conditions. Finally, the Rsa I restriction enzyme produced a species-specific pattern which allowed the identification of all the isolates as S. cerevisiae .
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