Gonadotropin stimulation of testosterone production in primary culture of adult rat testis cells |
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Authors: | A J Hsueh |
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Institution: | Department of Reproductive Medicine University of California, San Diego La Jolla, California 92093 USA |
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Abstract: | Testis cells from adult hypophysectomized rats were cultured in serum-free medium. Treatment with human chorionic gonadotropin caused an initial increase and a subsequent decline in testosterone production, followed by a recovery in steroidogenesis on day 10 of culture. The recovery in testosterone production was inhibited by the addition of serum in culture media. Luteinizing hormone, dibutyryl adenosine-3′,5′-monophosphate or cholera toxin, but not follicle stimulating hormone or prolactin, stimulated testosterone production which was potentiated by a phosphodiesterase inhibitor. This is the first report of a primary culture of adult testis cells with retention of androgen synthetic capacity. |
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