Using the PCR-RFLP method for comparative analysis of the pathogenic Escherichia coli strains

The purpose of the study was to identify differences and similarities between Escherichia coli strains which do or do not utilize disaccharide sucrose by PCR-RFLP method. Investigations were done on chromosomal DNA level using cscA gene associated with conservative sequences. The cscA gene may be found in all of the analysed strains. Genotypic analysis demonstrated presence of the same restriction model consisted of 2 DNA fragments with size of 161 bp and 110 bp in all of E. coli strains. Results of these investigations have shown that there are no differences between E. coli strains.