多种植物挥发物及马铃薯甲虫聚集素对马铃薯甲虫的引诱作用

马铃薯甲虫Leptinotarsa decemlineata (Say)是马铃薯上一种最主要的毁灭性害虫。由于其严重的抗药性， 急需寻找一种替代杀虫剂的防治方法。本实验从开发马铃薯甲虫引诱剂的目的出发，用“Y”型嗅觉仪测定了马铃薯甲虫对来自植物的7种挥发物单体、8个挥发物混合物配方以及马铃薯甲虫聚集素的行为反应，并进一步进行了田间诱集试验。室内生测结果表明，2-苯乙醇对马铃薯甲虫雌雄虫都有明显的引诱作用。芳樟醇+水杨酸甲酯+顺乙酸-3-己烯酯的混合物对马铃薯甲虫雄虫有很强的引诱作用(81.67%)， 但是对雌虫引诱作用不明显(63.33%)。在其中加入马铃薯甲虫聚集素不但提高了对雄虫的引诱率（88.33%），还消除了之前的雌雄性别间反应差异现象，使对雌虫的引诱率达到了83.33%。田间诱集结果显示，芳樟醇+水杨酸甲酯+顺乙酸-3-己烯酯+马铃薯甲虫聚集素在所研究的引诱剂配方中引诱效果最好。从而为马铃薯甲虫发生的预测预报和诱杀技术提供了研究基础。

Attraction of Leptinotarsa decemlineata (Say)(Coleoptera: Chrysomelidae) by several plant volatiles and aggregation pheromone

The Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say), is one of the most notorious insect pests of potato. Because of its serious resistance to pesticides, an alternative control of CPB is desperately needed. In order to develop an effective attractant for trapping CPB, the behavioral responses of CPB to seven plant-derived volatiles, eight odorous blends, and CPB aggregation pheromone were tested by the Y-tube olfactometer. Field trapping was further conducted based on the results of laboratory behavioral bioassays. The results of laboratory behavioral bioassays showed that 2-phenylethanol was attractive to both sexes. While male beetles were strongly attracted to a three-component blend ［comprised of (Z)-3-hexenyl acetate + linalool + methyl salicylate］ (81.67%), females showed low attraction (63.33%). The addition of aggregation pheromone to the three-component blend increased the attraction percentage of both male (88.33%) and female beetles (83.33%), and reduced the difference between the responses of sexes. The results of field tests indicated that the combination of the pheromone and the blend of three-component plant volatiles exhibited the highest attraction to CPB in the field. So these results provide a basis for the use of the pheromone and plant volatiles as a component of forecast and control of the CPB.