Agrobacterium-mediated transformation of strawberry calli and recovery of transgenic plants |
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| Authors: | Narender S Nehra Ravindra N Chibbar Kutty K Kartha Raju S S Datla William L Crosby Cecil Stushnoff |
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| Institution: | (1) Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Road, Saskatoon, S7N OW9 Saskatchewan, Canada;(2) Department of Horticulture Science, University of Saskatchewan, Saskatoon, S7N OWO Saskatchewan, Canada;(3) Present address: Department of Biochemistry, Colorado State University, Fort Collins, CO, USA |
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| Abstract: | Transformed calli and shoots of strawberry (Fragaria × ananassa Duch.) cv. Redcoat were obtained using Agrobacterium tumefaciens carrying plasmid pB1121. Inoculated leaf explants produced transgenic calli at a frequency of 3% on selection medium containing 50  g/ml kanamycin. Twenty per cent of selected caili regenerated, giving rise to transgenic shoots. All transgenic calli and shoots expressed substantial amounts of GUS and NPT-II activity. The Southern blot analysis confirmed the insertion of both marker genes into the strawberry genome as single and multiple copy inserts. The transgenic shoots elongated on rooting medium in the presence of 25 g/ml kanamycin, but exhibited reduced rooting ability.Abbreviations BA
benzyladenine
- NAA
1-naphthaleneacetic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid
- IBA
indole-3-butyric acid
- NPT-II
neomycin phosphotransferase(EC 2.7.1.95)
- GUS
 -glucuronidase(EC 3.2.1.31)
- X-GLUC
5-bromo-4-chloro-3-indolyl-glucuronide
- 4-MU
4-methylumbelliferone
NRCC No. 31227 |
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