| 噬菌体phi29 pRNA载体在RNA干扰中的初步应用研究 |
| |
| 引用本文： | 田拥军,张正茂,杨燕,覃莉,孟忠吉,刘慎沛,杨东亮.噬菌体phi29 pRNA载体在RNA干扰中的初步应用研究[J].病毒学报,2007,23(2):148-152. |
| |
| 作者姓名： | 田拥军 张正茂 杨燕 覃莉 孟忠吉 刘慎沛 杨东亮 |
| |
| 作者单位： | 华中科技大学,同济医学院附属同济医院临床免疫研究室,湖北,武汉,430030;华中科技大学同济医学院附属同济医院实验医学研究中心,湖北,武汉,430030;华中科技大学,同济医学院附属同济医院临床免疫研究室,湖北,武汉,430030;华中科技大学同济医学院附属同济医院实验医学研究中心,湖北,武汉,430030 |
| |
| 基金项目： | 国家自然科学基金资助项目（30571646）;国家教育部博士学科点科研基金（20030487070） |
| |
| 摘 要： | RNA干扰是在细胞胞质中双链RNA(dsR-NA)介导的序列特异性mRNA的降解1]。这个过程是由21~25个被称为小干扰RNA(si RNA)形成的dsRNA完成2]。目前,这一技术已经广泛应用于研究基因的功能,病毒感染治疗等方面。但是,si RNA在体内容易降解,干扰作用持续的时间不长。新的研究表明枯
|
| 关 键 词： | 噬菌体 pRNA RNA干扰 乙型 肝炎病毒 |
| 文章编号： | 1000-8721（2007）02-0148-05 |
| 修稿时间： | 2006-08-092006-11-20 |
Bacteriophage phi29 pRNA as Small Interference RNA Vector in RNA Interference |
| |
| TIAN Yong-jun,ZHANG Zheng-mao,YANG Yan,QIN Li,MENG Zhong-ji,LIU Shen-pei,YANG Dong-liang.Bacteriophage phi29 pRNA as Small Interference RNA Vector in RNA Interference[J].Chinese Journal of Virology,2007,23(2):148-152. |
| |
| Authors: | TIAN Yong-jun ZHANG Zheng-mao YANG Yan QIN Li MENG Zhong-ji LIU Shen-pei YANG Dong-liang |
| |
| Institution: | 1. Division of Clinical Immunology, and 2. Research Center of Experimental Medicine of Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China |
| |
| Abstract: | Gene-silencing siRNA has shown great promise for the treatment of genetic diseases,cancers,and viral infections,but its therapeutic value has been hindered by the lack of an efficient and safe in vivo delivery system to target specific cells.The motor pRNA of phi29 has a strong tendency to form dimers,trimers and hexamers by interaction of interlocking right and left hand loops.This unique feature makes pRNA an ideal vector for the delivery of multiple therapeutic RNAs.Toxicity of pRNA was detected by transfection of 8 pRNA in HeLa cells.A pRNA-based vector was designed to carry siRNAs to inhibit GFP or HBV surface gene expression.Silence effects on siRNA against expression of GFP or HBV surface gene were detected in HeLa cells.Viral replicative intermediates were detected by Southern blotting.The results of toxicity study showed there was no toxicity of pRNA to cultured monolayer cells.The siRNA connected with pRNA can inhibit GFP or HBV surface gene expression in HeLa cells and inhibit HBV replication in HepG2 cells.These data suggest that pRNA can be used as a vector for imparting stability to siRNA in vitro. |
| |
| Keywords: | bacteriophage vector RNA interference hepatitis B virus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
