Properties of a sucrose-tolerant Mutant of Saccharomyces cerevisiae |
| |
Authors: | Takahiro Oba Hikaru Suenaga Shigeru Muta Kosuke Tashiro Satoru Kuhara |
| |
Affiliation: | (1) Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa-machi, Kurume, Fukuoka 839-0861, Japan;(2) Graduate School of Genetic Resources Technology, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan |
| |
Abstract: | We characterized a sucrose-tolerant mutant of Saccharomyces cerevisiae, S22, that produces about four times as much acetate as the wild-type strain K9. We monitored the concentration of extracellular acetate during cultivation, and compared the gene expression ratios of S22 with those of K9 using DNA microarray. We propose that the sucrose tolerance of S22 may be related to the overexpression of the ENA1, ENA2, and ENA5 genes and some cell wall mannoprotein genes, and that the high acetate productivity of S22 is related to the overexpression of the ALD4 gene and oxidative phosphorylation genes. |
| |
Keywords: | Acetate DNA microarray analysis Saccharomyces cerevisiae Sake yeast Sucrose-tolerance |
本文献已被 SpringerLink 等数据库收录! |
|