Lipid response patterns in acute phase paediatric <Emphasis Type="Italic">Plasmodium falciparum</Emphasis> malaria |
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Authors: | Judy Orikiiriza Izabella Surowiec Elisabeth Lindquist Mari Bonde Jimmy Magambo Charles Muhinda Sven Bergström Johan Trygg " target="_blank">Johan Normark |
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Institution: | 1.Infectious Diseases Institute, College of Health Sciences,Makerere University,Kampala,Uganda;2.Department of Immunology, Institute of Molecular Medicine, Trinity College Dublin,St. James’s Hospital,Dublin,Ireland;3.Rwanda Military Hospital,Kigali,Rwanda;4.Computational Life Science Cluster (CLiC), Department of Chemistry,Ume? University,Ume?,Sweden;5.Department of Molecular Biology,Ume? University,Ume?,Sweden;6.Department of Chemistry,Ume? University,Ume?,Sweden;7.Department of Immmunology and Microbiology, School of Biomedical Sciences College of Health Sciences,Makerere University,Kampala,Uganda;8.Laboratory for Molecular Infection Medicine Sweden (MIMS),Ume?,Sweden;9.Ume? Center for Microbial Research,Ume?,Sweden;10.Division of Infectious Diseases, Department Clinical Microbiology,Ume? University,Ume?,Sweden |
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Abstract: | IntroductionSeveral studies have observed serum lipid changes during malaria infection in humans. All of them were focused at analysis of lipoproteins, not specific lipid molecules. The aim of our study was to identify novel patterns of lipid species in malaria infected patients using lipidomics profiling, to enhance diagnosis of malaria and to evaluate biochemical pathways activated during parasite infection.MethodsUsing a multivariate characterization approach, 60 samples were representatively selected, 20 from each category (mild, severe and controls) of the 690 study participants between age of 0.5–6 years. Lipids from patient’s plasma were extracted with chloroform/methanol mixture and subjected to lipid profiling with application of the LCMS-QTOF method.ResultsWe observed a structured plasma lipid response among the malaria-infected patients as compared to healthy controls, demonstrated by higher levels of a majority of plasma lipids with the exception of even-chain length lysophosphatidylcholines and triglycerides with lower mass and higher saturation of the fatty acid chains. An inverse lipid profile relationship was observed when plasma lipids were correlated to parasitaemia.ConclusionsThis study demonstrates how mapping the full physiological lipid response in plasma from malaria-infected individuals can be used to understand biochemical processes during infection. It also gives insights to how the levels of these molecules relate to acute immune responses. |
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