Cloning, expression and chromosomal location of NKX6B to 10q26, a region frequently deleted in brain tumors |
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Authors: | Seung-Hwan Lee John A Davison Silvia M Vidal Abdelmajid Belouchi |
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Institution: | (1) Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, Canada, K1H 8M5, CA;(2) Mycota Biosciences Inc., 225 President Kennedy Ave. W, suite 2550, Montréal, Québec, Canada, H2V 3Z6, CA;(3) Galileo Genomics Inc., 6700, avenue du Parc bureau 200, Montréal, Québec, Canada, H2V 4H9, CA |
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Abstract: | Nkx6-2 (former Gtx) is a murine-homeobox-containing gene localized distally on Chromosome (Chr) 7. Analysis of the expression pattern, together
with DNA binding assays, suggests that this gene product might be important for differentiated oligodendrocyte function and
in the regulation of myelin gene expression. We now report on the cloning and characterization of the human homolog (NKX6B).
DNA sequence analysis of an 11-kb genomic fragment revealed that the complete human gene spans 1.2 kb and is composed of three
exons. NKX6B is predicted to encode a polypeptide of 277 amino acids with 97% identity to mouse Nkx6-2. Northern blot experiments showed that NKX6B expression is tightly controlled in a tissue-specific fashion with the highest
site of expression being the brain. Finally, using STS content mapping and RH analyis, we demonstrated that NKX6B maps to
the 10q26, a region where frequent loss of heterozygosity has been observed in various malignant brain tumors. These results
may implicate NKX6B as a candidate tumor suppressor gene for brain tumors, particularly for oligodendrogliomas.
Received: 14 June 2000 / Accepted: 21 September 2000 |
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