产二十二碳六烯酸等多不饱和脂肪酸真菌的筛选*

从土壤中筛选出一株产二十二碳六烯酸（DHA）的丝状真菌，菌丝含油21.23％，DHA占总脂肪酸2.51％；同时含二十碳五烯酸（EPA），占总脂肪酸的0．41％；不饱和脂肪酸占总脂肪酸的80％。经鉴定为头孢霉属（Caphalosporiumsp．）真菌。同时发现两株菌含EPA，经鉴定为小克银汉霉（Cunninghamellasp.）和毛霉（Mucorsp．）。在这几个属中发现DHA和EPA尚属首次。头孢霉菌DHA产量及百分含量和斜面菌种在不同温度下储藏有关。菌种在20℃储藏10天，在液体PDA培养基上发酵，DHA可占总脂肪酸11．27％，产量达63．35mg／L。

THE SCREENING AND IDENTIFICATION OF FUNGI CONTAINING POLYUNSATURATED FATTY ACID

A strain of Cephaiosporium sp which produced docosahexaenoic acid (DHA) was screened. It contained 21.23% oil in its dry cell.The DHA made up 2.51% of the total fatty acid and the eicosapentaenoic acid (EPA) 0.41%.The unsaturated fatty acid made up 80% of the total fatty acid. A strain of Cunningamella sp. and a strain of Mucor sp. containing DHA and EPA were also found for the first hme.The yield of DHA in mycelium of Cephalosporium sp.was related to culture temperature.At 20℃ for 10 days, the yield of DHA reaches 63.35mg/L and amount to 11.27% of the total fatty acid.