Participation of the mitochondrial permeability transition pore in nitric oxide-induced plant cell death |
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Authors: | Elzira E Saviani Cintia H Orsi Jusceley F P Oliveira Cecília A F Pinto-Maglio Ione Salgado |
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Institution: | Departmento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, SP, Brazil. |
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Abstract: | In the present study, we investigated the involvement of the mitochondrial permeability transition pore (PTP) in nitric oxide (NO)-induced plant cell death. NO donors such as sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine inhibited growth and caused death in suspension-cultured cells of Citrus sinensis. Cells treated with SNP showed chromatin condensation and fragmentation, characteristic of apoptosis. SNP caused loss of the mitochondrial membrane electrical potential, which was prevented by cyclosporin A (CsA), a specific inhibitor of PTP formation. CsA also prevented the nuclear apoptosis and subsequent Citrus cell death induced by NO. These findings indicate that mitochondrial PTP formation is involved in the signaling pathway by which NO induces apoptosis in cultured Citrus cells. |
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Keywords: | Nitric oxide Programmed cell death Mitochondrial permeability transition pore Apoptosis Citrus cell |
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