Adhesion and colonization of enterohemorrhagic Escherichia coli O157:H7 in cecum of mice |
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Authors: | Nagano Keiji Taguchi Kazuki Hara Takumi Yokoyama Shin-ichiro Kawada Kenji Mori Hiroshi |
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Institution: | Laboratory of Microbiology, Department of Public Health Pharmacy, Gifu Pharmaceutical University, Gifu, Gifu 502-8585, Japan. |
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Abstract: | Infectious diseases due to enterohemorrhagic Escherichia coli (EHEC) are characterized by diarrhea, hemorrhagic colitis and hemolytic uremic syndrome. The adherence of EHEC on intestinal epithelial cells is a first step for developing these diseases. In the present study, we examined whether EHEC O157:H7 adhere to intestinal epithelial cells of mice and cause F-actin accumulation in the epithelial cells following the intragastric inoculation of the pathogen. Fecal shedding of the EHEC O157:H7 strain was observed in ICR mice up to 3 weeks. Fecal shedding periods of the type III secretion system-related gene (espA and sepL) deletion mutants were clearly shorter than that of the wild-type EHEC O157:H7 strain. The EHEC O157:H7 colonies were found on the epithelial surfaces of the ceca in association with F-actin accumulation beneath the attached bacteria. |
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Keywords: | EHEC O157:H7 mice adhesion colonization |
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