Optimization of protein extraction from Gelidiella acerosa by carbohydrases using response surface methodology |
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| Authors: | Majideh Jamshidi Javad Keramat Nasser Hamdami Omidvar Farhadian |
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| Institution: | 1. Department of Food Science and Technology, College of Agriculture, Isfahan University of Technology, Isfahan, Iran;2. Department of Natural Resources, Isfahan University of Technology, Isfahan, Iran |
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| Abstract: | In this study, application of response surface methodology for enzymic pretreatment optimization of Gelidiella acerosa was investigated in order to improve the extraction of algal proteins using Viscozyme L and Celluclast 1.5L. The total protein, soluble proteins and reducing sugar recovery in the water‐soluble fraction were studied in relation to the hydrolysis time, type and concentration of the enzymes. Enzymatic digestion appeared to be an effective treatment for protein extraction. While enzyme hydrolysis by Celluclast 1.5L was able to facilitate the protein extraction, it was a relatively inefficient way to improve protein extraction yield, in comparison with Viscozyme L. The optimum conditions for protein extraction was found to be hydrolysis by 2.8 μL mL?1 of Viscozyme L for 12 h. |
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| Keywords: | Celluclast 1 5L extraction of protein macroalgae Viscozyme L |
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