Investigation of the heterogeneity of xyloglucans from the cell walls of apple |
| |
Authors: | Pilar Ruperez Robert R. Selvendran Barry J.H. Stevens |
| |
Affiliation: | C.S.I.C. Instituto de Inmunologia Y Biologia Microbiana, Velazquez, 144, Madrid-28006 Spain;AFRC Food Research Institute, Colney Lane, Norwich NR4 7UA Great Britain |
| |
Abstract: | Cell-wall material from parenchymatous tissues of apple was sequentially extracted with 50mm NaOH at 1°, m KOH at 1° and 20°, and 4m KOH at 20°, to leave a residue of α-cellulose. From the 4m KOH-soluble fraction, a crude xyloglucan was isolated by anion-exchange chromatography, and further resolved into seven xyloglucans by borate anion-exchange chromatography. The relative amounts of the xyloglucans, in order of elution, were 2.7:1.3:29.7:1.0:3.2:1.2:10.3. The structural features of five of the xyloglucans were determined by methylation analysis. These results show that apple xyloglucans exhibit heterogeneity. |
| |
Keywords: | To whom correspondence should be sent |
本文献已被 ScienceDirect 等数据库收录! |
|