Structural analysis of the xyloglucan from Phaseolus coccineus cell-walls using cellulase-derived oligosaccharides |
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Authors: | Malcolm A ONeill Robert R Selvendran |
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Institution: | AFRC Food Research Institute, Colney Lane, Norwich, NR4 7UA Great Britain |
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Abstract: | Oligosaccharides, obtained by digestion of a xyloglucan from the cell walls of Phaseolus coccineus with cellulase, have been isolated by gel filtration. Oligosaccharides containing 2–6 residues accounted for ~57% of the hydrolysate, with larger oligosaccharides (d.p. ~10) and partially degraded xyloglucan accounting for ~32% of the polymer. The major glycosidic linkages were determined by methylation analysis. Methylated penta- and hexa-saccharide alditols were isolated by reverse-phase h.p.l.c. and characterised by e.i.-m.s. and f.a.b.-m.s. Methylated derivatives of the di-, tri-, and tetra-saccharide alditols were examined by g.l.c.-m.s. in the e.i. and c.i. modes. A structure based on these results is proposed. |
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