| 基于异肽键连接的分子粘合剂研究进展 |
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| 引用本文： | 高德英,曹佳雯,孙小宝,周可鑫,张铁涛,王谦. 基于异肽键连接的分子粘合剂研究进展[J]. 生物工程学报, 2019, 35(4): 607-615 |
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| 作者姓名： | 高德英 曹佳雯 孙小宝 周可鑫 张铁涛 王谦 |
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| 作者单位： | 1 浙江万里学院 生物与环境学院，浙江 宁波 315100,1 浙江万里学院 生物与环境学院，浙江 宁波 315100;3 浙江大学 动物科学学院，浙江 杭州 310012,1 浙江万里学院 生物与环境学院，浙江 宁波 315100,1 浙江万里学院 生物与环境学院，浙江 宁波 315100,2 中国农业科学院 特产研究所，吉林 长春 130112,1 浙江万里学院 生物与环境学院，浙江 宁波 315100 |
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| 基金项目： | 国家级大学生创新创业训练计划 (No. 201710876020)，浙江省生物工程重中之重学科开放基金 (No. KF2016010) 资助。 |
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| 摘 要： | 异肽键分子粘合剂是多肽链中的赖氨酸(Lys)残基和天冬酰胺或天冬氨酸(Asn/Asp)残基侧链自发结合形成的不可逆共价键,具有良好的特异性和稳定性。该反应可在极短的时间完成,且不需要特定的理化环境和催化剂。文中结合近年来国内外学者对异肽键分子粘合剂的研究进展,综述了异肽键分子粘合剂的来源、类型、形成机制及其介导的分子环化和蛋白拓扑结构,并讨论了其在合成疫苗、水凝胶和细菌纳米生物反应器等领域的应用前景。
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| 关 键 词： | 异肽键，分子环化，机制，SpyTag/SpyCatcher，应用 |
| 收稿时间： | 2018-07-20 |
Advances in isopeptide bond-mediated molecular superglue |
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| Deying Gao,Jiawen Cao,Xiaobao Sun,Kexin Zhou,Tietao Zhang and Qian Wang. Advances in isopeptide bond-mediated molecular superglue[J]. Chinese journal of biotechnology, 2019, 35(4): 607-615 |
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| Authors: | Deying Gao Jiawen Cao Xiaobao Sun Kexin Zhou Tietao Zhang Qian Wang |
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| Affiliation: | 1 College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China,1 College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China;3 College of Animal Sciences, Zhejiang University, Hangzhou 310012, Zhejiang, China,1 College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China,1 College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China,2 Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, Jilin, China and 1 College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China |
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| Abstract: | Isopeptide bond-mediated molecular superglue is the irreversible covalent bond spontaneously formed by the side chains of lysine (Lys) and asparagine/aspartic acid (Asn/Asp) residues. The peptide-peptide interaction is specific, stable, and can be achieved quickly without any particular physicochemical factor. In the light of recent progress by domestic and foreign researchers, here we summarize the origin, assembly system and mechanism of isopeptide bond reaction, as well as the molecular cyclization and protein topological structure mediated by it. The prospect for its application in synthetic vaccine, hydrogel and bacterial nanobiological reactor is further discussed. |
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| Keywords: | isopeptide bond molecular cyclization mechanism SpyTag/SpyCatcher application |
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