Recognition of Pyrimidine Dimers in DNA by the Incision Enzyme from <Emphasis Type="Italic">Micrococcus lysodeikticus</Emphasis> |
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Authors: | V P PARIBOK N V TOMILIN |
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Institution: | 1.Institute of Cytology of the Academy of Sciences of the USSR,Leningrad |
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Abstract: | A SIGNIFICANT proportion of the number of pyrimidine dimers induced in DNA by ultraviolet light is repaired by means of excision-resynthesis1–3. Two enzymes that excise pyrimidine dimers from DNA have been purified from cells of a highly ultraviolet-resistant microorganism—Micrococcus lysodeikticus4,5. One of these—an endonuclease—seems to recognize dimers and splits a phosphodiester bond near the dimers in DNA. The mechanism of recognition is not known; in particular, whether the incision enzyme recognizes either a local melting of DNA double helix or a specific chemical modification of one of DNA strands. If the former is correct, the incision enzyme should break the strand opposite to the dimer6 and the incision step in repair may lead to mutations6 or chromosome aberrations7. |
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