Formation of anti-plant viral protein by Mirabilis jalapa L. cells in suspension culture |
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Authors: | Tsutomu Ikeda Youichi Takanami Seiko Imaizumi Takashi Matsumoto Youichi Mikami Susumu Kubo |
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Institution: | (1) Central Research Institute, Japan Tobacco Inc., 6-2 Umegaoka, Midori-ku, 227 Yokohama, Japan |
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Abstract: | The extract of Mirabilis jalapa cultured cells and its precipitate fraction with 90% saturated ammonium sulfate showed an anti-plant viral activity comparable to that of the roots and leaves of the original plant. In the immunodiffusion experiment, the extract of cultured cells positively reacted with MAP (Mirabilis Anti-plant viral Protein) anti-serum. The changes in MAP formation during cell growth and the MAP content of roots and leaves were examined using enzyme-linked immunosorbent assay (ELISA). MAP formation proceeded almost in parallel with cell growth. The MAP content of cultured cells reached the highest level (0.6 mg/g dry weight) on the 9th day after inoculation, which was less than one-third of the content of the roots but three times larger than that of the leaves.Abbreviations MAP
Mirabilis anti-plant viral protein
- TMV
tobacco mosaic virus
- 2,4-D
2,4-dichlorophenoxyacetic acid
- ELISA
enzyme-linked immunosorbent assay
Studies on the production of anti-plant viral substances of higher plant cells in suspension culture. Part 1 |
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