Determination of H(2)S solubility via the reaction with ferric hemoglobin I from the bivalve mollusc Lucina pectinata |
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| Authors: | Boffi A Rizzi M Monacelli F Ascenzi P |
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| Affiliation: | CNR, Centro di Biologia Molecolare and Dipartimento di Scienze Biochimiche 'Alessandro Rossi Fanelli', Università di Roma 'La Sapienza', Rome, Italy. |
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| Abstract: | A new, simple and fast spectrophotometric method for the determination of the H(2)S concentration is reported. This method, based on the 1:1 reaction between H(2)S and the ferric derivative of hemoglobin I (HbI) from the bivalve mollusc Lucina pectinata, allows the quantitative determination of H(2)S dissolved in a given solution even at concentrations as low as 1 x 10(-6) M. Note that L. pectinata HbI is considered the physiological receptor of H(2)S. |
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