High cell density fermentation of <Emphasis Type="Italic">Gluconobacter oxydans</Emphasis> DSM 2003 for glycolic acid production |
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Authors: | Guodong Wei Xuepeng Yang Tula Gan Wenyu Zhou Jinping Lin Dongzhi Wei |
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Institution: | (1) State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 200237 Shanghai, China;(2) College of Food and Biological Engineering, Zhengzhou University of Light Industry, 450002 Zhengzhou, China |
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Abstract: | Gluconobacter oxydans has a lower biomass yield. Uniform design (UD) was applied to determine the optimum composition of the critical media and
their mutual interactions for increased biomass yield of Gluconobacter oxydans DSM 2003 in shake flasks. Fed-batch fermentation process for biomass was optimized in a 3.7-l fermentor. By undertaking a
preliminary and improved fed-batch fermentation-process strategy, a cell density of 6.0 g/l (DCW) was achieved in 22 h and
14.1 g/l (DCW) in 35 h, which is the highest cell density of G. oxydans produced thus far in a 3.7-l bioreactor. The biomass production was increased by 135% compared with that using the original
cultivation strategy. Bioconversion of ethylene glycol to glycolic acid was catalyzed by the resting cells of G. oxydans DSM 2003, and conversion rate reached 86.7% in 48 h. In summary, the approach including high-density fermentation of G. oxydans DSM 2003 and bioconversion process was established and proved to be an effective method for glycolic acid production. |
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Keywords: | Gluconobacter oxydans Uniform design Fed-batch High-density fermentation Bioconversion Ethylene glycol Glycolic acid |
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