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Development of buffers for fast semidry transfer of proteins |
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| Authors: | Dušan Garić Laure Humbert Nadège Fils-Aimé Juliana Korah Yasaman Zarfabian Jean-Jacques Lebrun Suhad Ali |
| Institution: | 1. Division of Hematology, Department of Medicine, Royal Victoria Hospital, McGill University Health Centre, McGill University, Montreal, QC H3A 1A1, Canada;2. Division of Medical Oncology, Department of Medicine, Royal Victoria Hospital, McGill University Health Centre, McGill University, Montreal, QC H3A 1A1, Canada |
| Abstract: | Western blot is an extensively used method for protein detection in cell biology. To optimize this procedure, here we examined a panel of buffers for their ability to efficiently transfer proteins from SDS–polyacrylamide gels onto nitrocellulose membranes in a short 12-min period, designated here as fast semidry transfer. Our results show for the first time that HEPES- and HEPPS/EPPS-based buffers represent the most efficient buffers for fast semidry transfer. |
| Keywords: | Western blot Protein transfer HEPES HEPPS |
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