Preparation of 2-nitro-5-thiobenzoate for the routine determination of reagent hypochlorous acid concentrations |
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Authors: | Thomas M. Jeitner Mike KalogiannisJim Mathew |
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Affiliation: | Winthrop University Hospital, Mineola, NY 11501, USA |
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Abstract: | Solutions of hypochlorous acid (HOCl) decay over time. This decay indicates the necessity for methods and reagents for the routine measurement of this oxidant. 2-Nitro-5-thiobenzoate is commonly used to measure HOCl concentrations. This article describes a method for the preparation of 2-nitro-5-thiobenzoate that is stable for at least 3 months. This method relies on the partial rather than full reduction of 5,5′-dithiobis-(2-nitrobenzoic acid) and the resulting equilibrium between the substrate and the product. |
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Keywords: | Hypochlorous acid 2-Nitro-5-thiobenzoate Ellman&rsquo s reagent |
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